In vitro study of protective effect of Pterocarpus erinaceus Poir. stem bark and Amaranthus spinosus L. root extracts on cataractogenesis and glomerulopathy

Abstract Background Cataracts and glomerulopathy are disabling complications of diabetes mellitus. The use medicinal plants in the treatment helps prevent or delay these complications. Pterocarpus erinaceus Amaranthus spinosus part used traditional medicine to treat its aim this study was evaluate vitro protective effect dried hydroethanolic extracts two against cataractogenesis induced by high glucose. Lenses kidney fragments from Sprague–Dawley rats were cultured artificial aqueous humor glomerular solution under glucose, respectively. at doses 250 500 mg/kg bw added culture medium incubated for 72 h lenses 96 fragments. Morphological biochemical parameters evaluated during cultures. Results treated with both remained shiny transparent contrast opacity observed toxic controls. extract caused a significant ( p < 0.001) decrease malondialdehyde increase glutathione catalase levels also 0.0001) glucose absorption glomeruli tubular reabsorption This led incubation medium. Conclusion These findings showed that P. A. can be reduce hyperglycemia effects inhibiting oxidative stress pathways then preventing delaying onset cataracts failure diabetes.